BAFF (TNFSF13B) Rabbit Monoclonal Antibody [Clone ID: Tabalumab]

Specifications

Product Data
Clone Name	Tabalumab
Applications	ELISA, FC, FN, IF, IP, Neutralize, WB
Reactivity	Human, Monkey, Rabbit
Host	Rabbit
Clonality	Monoclonal
Immunogen	Monoclonal antibodies were generated by immunizing human immunoglobulin transgenic mice with soluble human BAFF (amino acids 133–285). Eight mice producing serum antibody titers to human BAFF were injected intravenously with 10 µg human BAFF in phosphate buffered saline. The spleen was harvested 3 days later from each mouse and fused with myeloma cells. 20 Hybridomas were tested for specific binding to human BAFF and to make sure they were expressing human immunoglobulin heavy and light chains by enzyme-linked immunosorbent assay (ELISA). The hybridoma producing the antibody that eventually became tabalumab was a human IgG1 isotype. The antibody was then engineered to a human IgG4 isotype for complete neutralization of BAFF without depletion of BAFF-expressing cells.
Specificity	Tabalumab neutralises soluble and membrane-bound BAFF, inhibiting BAFF binding to BR3, TACI and BCMA. Tabalumab has been tested in patients with systemic lupus erythematosus (Merrill 2015) and in patients with active rheumatoid arthritis with an inadequate response to TNF inhibitors (Genovese 2013).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	tumor necrosis factor superfamily member 13b
Database Link	Entrez Gene 10673 Human UniProt ID Q9Y275
Synonyms	BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; ZTNF4
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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