MHC I Rabbit Monoclonal Antibody [Clone ID: R1-9.6]

Specifications

Product Data
Clone Name	R1-9.6
Applications	IF, IP, R
Reactivity	Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
Specificity	This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody recognises a determinant distinct from those detected by most common mouse monoclonal anti-H-2 antibodies. This antibody binds murine H-2 MHC class I molecules, and has been used in both structural and biochemical studies. This antibody has been used in immunoprecipitation analysis (Robinson, 1987), and in immunofluorescence assays for MHC-I surface expression (Karacsonyi et al, 2004) and MHC-I endocytosis (Knorr et al, 2009).This antibody has also been employed to induce clustering of cell surface MHC-I in co-patching assays (Knorr et al, 2009). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells, and assess its strain specificity (Koch et al, 1983).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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