Rb (RB1) Rabbit Monoclonal Antibody [Clone ID: R01-8H7]

Specifications

Product Data
Clone Name	R01-8H7
Applications	IP, WB
Recommend Dilution	WB: 1/1000 IP: 1/20
Reactivity	Human, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Rb (Phosphorylated)
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 106 kDa; Observed MW: 110 kDa
Gene Name	RB transcriptional corepressor 1
Database Link	Entrez Gene 5925 Human UniProt ID P06400
Background	Swiss-Prot Acc.P06400.Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex .
Synonyms	OSRC; osteosarcoma; p105-Rb; pp110; pRb; RB
Reference Data

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