Cpn10 (HSPE1) Rabbit Monoclonal Antibody [Clone ID: R02-3K6]

Specifications

Product Data
Clone Name	R02-3K6
Applications	IHC, IP, WB
Recommend Dilution	WB: 1/2000-1/10000 IHC: 1/50-1/200 IP: 1/20-1/50
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human Cpn10
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 11 kDa; Observed MW: 11 kDa
Gene Name	heat shock protein family E (Hsp10) member 1
Database Link	Entrez Gene 3336 Human UniProt ID P61604
Background	Swiss-Prot Acc.P61604.Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:7912672, PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Synonyms	CPN10; EPF; GROES; Hsp10
Reference Data

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