CDK4 Rabbit Monoclonal Antibody [Clone ID: R07-4G7]

Specifications

Product Data
Clone Name	R07-4G7
Applications	IF, WB
Recommend Dilution	WB: 1/1000-1/5000 ICC/IF: 1/100
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human Cdk4
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 34 kDa; Observed MW: 34 kDa
Gene Name	cyclin-dependent kinase 4
Database Link	Entrez Gene 1019 Human UniProt ID P11802
Background	Swiss-Prot Acc.P11802.Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Synonyms	CMM3; MGC14458; PSK-J3
Reference Data

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