Hcrt Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against Orexin B. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln- Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2 coupled to carrier protein.
Formulation	This polyclonal antibody is supplied as a lyophilized powder. The powder should be rehydrated with 50ml RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4°C. This should ensure antibody stability for approximately one month.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	hypocretin
Database Link	Entrez Gene 15171 Mouse UniProt ID O55241
Background	Orexin B is a 28-amino acid neuropeptide hormone involved in the regulation a variety of behavioral and physiological processes in response to motivational stimuli. Orexins (Orexin A and Orexin B) are produced by proteolytic cleavage of prepro- orexin. They were so named for their stimulatory role in feeding behavior, but also play important functions in the organism's energy homeostasis and sleep cycles. They exert their effects through G-protein coupled receptors. This antibody was generated by immunization of rabbits with Orexin B coupled to a carrier protein.
Synonyms	Hypocretin; NRCLP1; orexin; OX; PPORX; PPOX
Reference Data

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