Spn Mouse Monoclonal Antibody [Clone ID: WEN3]

Specifications

Product Data
Clone Name	WEN3
Applications	IHC
Recommend Dilution	Each lot of this antibody has been tested and validated for immunohistochemistry (IHC); the antibody has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 0.1µg/ml (1:4000) Paraffin sections: 5µg/ml (1:80); microwave pretreatment for antigen retrieval is recommended. Optimal dilutions should be determined by the end user. Suggested positive control: rat spleen.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	rat lymphokine activated killer (LAK) cells
Specificity	Rat: subpopulations of CD4+, CD8+ T-cells. Other: does not react with porcine tissue. Epitope: The antigen has been identified as rat CD43, the epitope has not been characterized. Distribution: Tissue sections: Cells in T-cell areas mostly show a weak reaction, whereas strong staining is seen in scattered splenic cells in the red pulp.
Formulation	Affinity purified from cell culture supernatant, lyophilized, packaged under nitrogen. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Minimize repeated thawing and freezing of the stock solution.
Gene Name	sialophorin
Database Link	Entrez Gene 24796 Rat UniProt ID P13838
Background	Monoclonal antibody WEN3 reacts with rat CD43, also known as Leukosialin, Sialophorin or W3/13 antigen. CD43 is a major glycoprotein of thymocytes and T lymphocytes, a factor in lectin binding and counter-receptor for Siglec-1 (Sialoadhesin). CD43 is a single-pass type I membrane protein with a 371 amino acid backbone that presents carbohydrate ligands to selectins. T cells express two glycoforms of CD43, with molecular weights of 115 kDa and 130 kDa due to different glycosylation patterns. This antibody was produced in vitro from cell culture supernatant.
Synonyms	CD43; Galactoglycoprotein; GALGP; GPL115; leukosialin; LSN; Sialophorin
Reference Data

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