Pdpn Monoclonal Antibody [Clone ID: 36899]

Specifications

Product Data
Clone Name	36899
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 0.125-0.25µg/ml (1:800-1:1600) Paraffin sections: 1µg/ml (1:100); microwave pretreatment for antigen retrieval is recommended. Optimal dilutions should be determined by the end user. Suggested positive control: Mouse kidney.
Reactivity	Mouse
Clonality	Monoclonal
Immunogen	Thymic stromal cell line 186.
Specificity	Mouse: Podoplanin. Other species: The antibody does not react with swine tissues. Epitope: The epitope has not been further characterized. Distribution: Tissue Sections: The antigen is expressed on lymphatic endothelial cells, a subpopulation of thymic epithelial cells, mesothelium of different organs, and renal podocytes.
Formulation	Affinity purified, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.1% Kathon as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Gene Name	podoplanin
Database Link	Entrez Gene 14726 Mouse UniProt ID Q62011
Background	Podoplanin is a prominent antigen of the kidney glomerular epithelial cell, the podocyte, where it seems to play a role in controlling glomerular permeability. Podoplanin is a mucin-type single-pass membrane protein, with its blocked N- terminus on the extracellular side. It contains 172 amino acids, is extensively O- glycosylated, and contains numerous sialic acid residues. Podoplanin is also a well- established lymphatic endothelial cell marker and as such a useful marker to distinguish lymphatic vessels immunohistochemically from blood vessels. High levels can be detected in lung and brain, lower levels in kidney, stomach, liver, spleen and esophagus, none in skin and small intestine. Some malignant tumor cells express podoplanin as well. The concomitant changes in cell morphology and motility suggest that podoplanin influences tumor progression. Podoplanin deficient mice die at birth because of respiratory failure. Podoplanin deficient mice also have defects in lymphatic formation with diminished lymphatic transport and congenital lymphedema.
Synonyms	aggrus; GP36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; OTS8; PA2.26; podoplanin; T1-alpha; T1A; T1A-2
Reference Data

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