Itgam Rat Monoclonal Antibody [Clone ID: M1/70]

Specifications

Product Data
Clone Name	M1/70
Applications	ELISA, IHC
Recommend Dilution	Each lot of this antibody has been tested and validated for immunohistochemistry (IHC), frozen and paraffin sections. Has also been described to work in FACS, western blotting and immune precipitation. Approximate working dilution for IHC: Frozen sections: 5µg/ml (1:100) Paraffin sections: does not react on routinely processed paraffin sections. Optimal dilutions should be determined by the end user. Suggested positive control: Mouse spleen.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	T-cell enriched splenocytes from B10 mice.
Specificity	Mouse: monocytes, macrophages, granulocytes, endothelial and NK cells. Other species: human. Epitope: The antigen is an epitope of the CD11b integrin on the αm chain. Distribution: Isolated cells: Granulocytes, monocytes: antigen expression increases with cell maturation.
Formulation	Affinity purified from ascitic fluid, lyophilized. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.5mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Original vial: 6 months at 4° - 8°C.
Gene Name	integrin alpha M
Database Link	Entrez Gene 16409 Mouse UniProt ID P05555
Background	Monoclonal antibody M1/70 is useful for detecting CD11b on myeloid cells and for myeloid- endothelial cell interaction studies. The antibody detects the 170kDa α subunit of Mac-1 which mediates adhesion to ICAM-1 (CD54) and C3bi. Mac-1 is expressed on granulocytes, macrophages, natural killer cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 is up-regulated on neutrophils after activation. This particular clone blocks cell adherence and C3bi binding but does not block cell mediated lysis. It has been used for the characterisation of LFA-1 immunodeficiency.
Synonyms	CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; SLEB6
Reference Data

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