Histiocytes Rat Monoclonal Antibody [Clone ID: ER-MP23]

Specifications

Product Data
Clone Name	ER-MP23
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 1µg/m (1:200) Paraffin sections: not tested. Optimal dilutions should be determined by the end user. Suggested positive control: Mouse skin.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse macrophage cell lines.
Specificity	Mouse: Macrophages of connective tissue Other: unknown Epitope: The antigen is a glutaraldehyde (0.05%) resistant 38kD single chain surface glycoprotein. Distribution: Isolated cells: The antigen is found on the surface of connective tissue macrophages and their precursor cells in bone marrow. Macrophage cell lines (J774-1.6, RAW309Cr.1, WR19M.1) corresponding to mature macrophages express high levels of the antigen . Tissue sections: The antigen is abundant on mature macrophages in connective tissues as present in the skin (dermis), salivary glands, capsule of lymph nodes, thymus and various other organs. The antigen is partly coexpressed with the BM8 antigen. ER-MP23 positive macrophages may express Ia antigens.
Formulation	Affinity purified, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Background	Monoclonal antibody ER-MP23 detects mature macrophages in connective tissues which are situated in the vicinity of epithelia e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. The antibody blocks the mouse macrophage galactose-specific lectin (MMGL).
Reference Data

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