EMAP II (AIMP1) Monoclonal Antibody [Clone ID: 546-2]

Specifications

Product Data
Clone Name	546-2
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in ELISA and Western Blot. Approximate working dilution for IHC: Frozen sections: 5µg/ml (1:40) Paraffin sections: 5-10µg/ml (1:20-1:40), microwave pretreatment for antigen retrieval is recommended. Optimal dilutions should be determined by the end user. Suggested positive control: rat spleen.
Reactivity	Human
Clonality	Monoclonal
Immunogen	Recombinant human EMAP II.
Specificity	Human: EMAP II Other species: rat. Epitope: The antigen is EMAP II, the epitope has not been further characterized.
Formulation	Affinity purified,biotinylated, lyophilized Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% Kathon CG as a preservative.
Concentration	N/A
Conjugation	Biotin
Storage Condition	Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Gene Name	aminoacyl tRNA synthetase complex interacting multifunctional protein 1
Database Link	Entrez Gene 9255 Human UniProt ID Q12904
Background	EMAP II is a proinflammatory cytokine and chemoattractant for macrophages. The 20kDa mature peptide binds to endothelial cells and leukocytes like macrophages. EMAP II activates endothelial cells, by elevation of cytosolic free calcium concentration, release of von Willebrand factor, induction of tissue factor, and expression of adhesion molecules E- selectin and P-selectin. It further activates resting monocytes by elevating cytosolic free Ca2+, inducing TNFα and tissue factor, and stimulating chemotaxis. EMAP II has been shown in a tumour model to sensitize the local tumour vasculature to the action of TNFα, rendering resistant tumours sensitive to immunotherapy by TNFα. This monoclonal antibody was obtained by immunisation with human EMAP II and reacts with rat tissues as well.
Synonyms	EMAP-2; EMAP2; EMAPII; p43; SCYE1
Reference Data

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