CTLA4 Mouse Monoclonal Antibody [Clone ID: 2G10]

Specifications

Product Data
Clone Name	2G10
Applications	ELISA, IF, IHC, WB
Recommend Dilution	WB: 1-2µg/mL.IHC starting at 5µg/mL.IF start at 20µg/mL.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
Formulation	CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	CTLA-4 Antibody is supplied as protein A purified IgG1.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 25 kDa; Observed: 32 kDa
Gene Name	cytotoxic T-lymphocyte associated protein 4
Database Link	NP_005205 Entrez Gene 1493 Human UniProt ID P16410
Background	The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).
Synonyms	CD; CD28; CD152; CELIAC3; CTLA-4; GRD4; GSE; ICOS; IDDM12
Note	CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Reference Data

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