GAPDH Mouse Monoclonal Antibody [Clone ID: 12D3D5]

Specifications

Product Data
Clone Name	12D3D5
Applications	WB
Recommend Dilution	WB: 1-2µg/ml.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
Formulation	GAPDH antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	GAPDH antibody is Protein A purified.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 37 kDa; Observed: 36 kDa
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	NP_002037 Entrez Gene 2597 Human UniProt ID P04406
Background	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Synonyms	G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432
Note	GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 µg/ml.
Reference Data

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