Vinculin (VCL) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	WB: 0.5-1µg/ml. IHC starting at 5µg/mL. IF start at 20µg/mL.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Vinculin antibody was raised against a 23 amino acid peptide near the amino of human Vinculin.
Formulation	Vinculin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	Vinculin antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 120 kDa; Observed: 117 kDa
Gene Name	vinculin
Database Link	NP_003364 Entrez Gene 7414 Human UniProt ID P18206
Background	Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Synonyms	CMD1W; metavinculin; MVCL; vinculin
Note	Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 µg/ml. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Reference Data

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