PAK4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide surrounding the epitope RKSLV-with a phosphorylation at Serine 474 of human PAK4. This sequence is also identical within human, rat, mouse, chicken, bovine and dog species.
Formulation	This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Purification	The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	68 kDa
Gene Name	p21 (RAC1) activated kinase 4
Database Link	NP_001014831 Entrez Gene 70584 MouseEntrez Gene 292756 RatEntrez Gene 10298 Human UniProt ID O96013
Background	p21-activated protein kinase (PAK) serine/threonine kinases are important effectors of Rho family GTPases and have been implicated in the regulation of cell morphology and motility, as well as in cell transformation. The phosphorylation of serine 474 as the likely autophosphorylation site in the kinase domain of PAK4 occurs in vivo. PAK4 is frequently over-expressed in human tumor cell lines of various tissue origins, indicates that the phosphorylation of PAK4 is required in oncogenic transformation and Ras-driven, anchorage-independent growth.
Synonyms	effector for Cdc42Hs; p21 protein (Cdc42; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; protein kinase related to S. cerevisiae STE20; Rac)-activated kinase 4
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway

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