GAPDH Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 2000-10000 WB positive control: Hela, 293T, Jurkat, A431, A549 cell lysates IHC: 50-200 Positive control: Human esophagus cancer Predicted cell location: Cytoplasm and Nucleus
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fusion protein of human GAPDH
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	37 kDa
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	NP_002037 Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human UniProt ID P04406
Background	The abundance of glyceraldehyde-3-phosphate dehydrogenase in eukaryotic cells is relatively unaffected by eternal factors. In western blot analysis the level of GAPDH, a 38kDa protein, can be used as a reference value for loading control. Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Synonyms	G3PD; GAPD; HEL-S-162eP
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

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