APAF1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:500-1:3000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen for anti-APAF1 Antibody: A synthesized peptide derived from human APAF1
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20?. Stable for 12 months from date of receipt
Concentration	lot specific
Purification	Affinity-chromatography
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	27 kDa
Gene Name	apoptotic peptidase activating factor 1
Database Link	NP_863659 Entrez Gene 317 Human UniProt ID O14727
Background	APAF Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Induced by E2F and p53 in apoptotic neurons. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. 6 isoforms of the human protein are produced by alternative splicing.
Synonyms	APAF-1; CED4
Note	APAF1 antibody detects endogenous levels of total APAF1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Huntington's disease, p53 signaling pathway, Parkinson's disease, Small cell lung cancer

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