Histone H2A.X (H2AFX) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry, Western Blot: 2-4ug/ml~, Immunohistochemistry-Paraffin: 10ug/ml
Reactivity	Human, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A portion of amino acids 100-153 of human Histone H2A.x was used as the immunogen for the antibody.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein A purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	H2A histone family member X
Database Link	NP_002096 Entrez Gene 3014 Human UniProt ID P16104
Background	H2A.x is a human histone H2A isoprotein containing 143 amino acid residues. H2A.x is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of H2A.x mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. H2A.x becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites. It is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. It maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
Synonyms	H2A; H2A.X; H2AX; X
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Systemic lupus erythematosus

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