EPS8 Mouse Monoclonal Antibody [Clone ID: 18F5B11]

Specifications

Product Data
Clone Name	18F5B11
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry, Immunohistochemistry-Paraffin: 5ug/ml, Western Blot: 1-5ug/ml~
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Amino acids 701-882 of human EPS8 protein were used as the immunogen for the EPS8 antibody.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	epidermal growth factor receptor pathway substrate 8
Database Link	NP_004438 Entrez Gene 2059 Human UniProt ID Q12929
Background	EPS8, an acronym for epidermal growth factor receptor pathways substrate 8, is a signaling protein and part of the molecular complex controlling actin reorganization (1, 2). EPS8 also plays an important role in innate immunity. Through LPS activation of TLR4 and the co adaptor MyD88 protein, macrophages induce enhanced EPS8 activity typified by phagocytosis- a key innate anti-microbial defense response (3). EPS8 colocalizes with TLR4 in LPS activated macrophage immunocomplexes (3). Interestingly, EPS8 also is over expressed in malignancies including breast cancers, pancreatic cancers, and colon cancers, as examples (2-4). Antibody to EPS8 provides a tool for characterizing its functional roles in motility, adhesion, signaling pathways such as non-canonical Wnt signaling and expression in normal and neoplastic cells (5).
Synonyms	DFNB102
Note	Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Reference Data
Protein Families	Druggable Genome

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