PDX1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Western Blot: 1.0 ug/ml, Immunohistochemistry-Paraffin: 1:200, Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:200 - 1:500
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of the human PDX1 protein (between residues 100-200) [UniProt P52945]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	pancreatic and duodenal homeobox 1
Database Link	NP_000200 Entrez Gene 18609 MouseEntrez Gene 3651 Human UniProt ID P52945
Background	PDX1, also known as IDX-1, IPF-1, STF-1, and MODY4 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. During development, PDX1 specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, it is required for maintaining the hormone-producing phenotype of the beta-cell. PDX1 is located in both the nucleus and cytosol on a cellular level, while in tissue it is distinct to the duodenum and pancreas. Defects in PDX1 gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). Recently, research of PDX1 ranges from an important role in the malaria parasite (PMID: 23039077) to mutations in PDX1 leading to neonatal diabetes (PMID: 20009086).
Synonyms	GSF; IDX-1; IPF1; IUF1; MODY4; PAGEN1; PDX-1; STF-1
Note	This PDX1 antibody is useful for Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. In Western blot a band is observed ~40 kDa, and in ICC/IF diffuse nuclear and cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
Protein Pathways	Maturity onset diabetes of the young, Type II diabetes mellitus

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