KDM5B Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Western Blot, Flow Cytometry, Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry-Paraffin: 1:200
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human JARID1B protein (between residues 800-850) [UniProt Q9UGL1]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	lysine demethylase 5B
Database Link	NP_006609 Entrez Gene 10765 Human UniProt ID Q9UGL1
Background	Jumonji AT-rich interactive domain 1B (JARID1B) also known as Jumonji/ARID domain-containing protein 1B, Lysine-specific demethylase 5B (KDM5B), Cancer/testis antigen 31 (CT31), Histone demethylase JARID1B, PLU-1, and Retinoblastoma-binding protein 2 homolog 1 (RBP2-H1), is a member of the JARID 1 family. The JARID 1 proteins (JARID1A, JARID1B, JARID1C and JARID1D) catalyze the demethylation of histones on lysine, and JARID1B specifically demethylates lysine 4 on Histone H3. JARID1B functions as a transcriptional co-repressor for FOXG1B and PAX9. In addition, JARID1B may play a considerable role in cancer research as it assists in the propagation of breast cancer cells by repressing the tumor suppressor genes BRCA1 and HOXA5. Conversely, JARID1B acts as a direct suppressor of melanoma. As result, the JARID1B protein is up regulated in breast cancer and down regulated in melanoma and glioblastomas. In general, JARID1B expression is ubiquitous; with the highest expression seen in testis. JARID1B is subject to post translational phosphorylation and has two known isoforms; isoform 1: 175.6kDa and isoform 2: 179.4kDa.
Synonyms	CT31; JARID1B; PLU-1; PLU1; PPP1R98; PUT1; RBBP2H1A
Note	This JARID1B antibody is useful for Flow Cytometry, Immunocytochemistryand paraffin embedded sections. In ICC/IF, staining of the nucleus was seen in HeLa cells. In paraffin, nuclear staining was observed in human breast cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in western blot reported in scientific literature ( PMID 23354547)
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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