LDHA Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Western Blot: 0.5ug/ml, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:50, Immunohistochemistry: 1:100, Flow Cytometry, ELISA, Immunohistochemistry-Frozen
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332). [Swiss-Prot# P00338]
Formulation	PBS, 30% glycerol, 0.1% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	36 kDa
Gene Name	lactate dehydrogenase A
Database Link	NP_005557 Entrez Gene 16828 MouseEntrez Gene 3939 Human UniProt ID P00338
Background	Lactate dehydrogenase (LDH) is a ubiquitous enzyme that catalyzes the inter-conversion of pyruvate and lactate, with simultaneous inter-conversion of NADH and NAD+. Mammalian LDH has five tetrameric isozymes comprising combinations of two subunits (i) A subunit (LDHA or LDHM) predominates in skeletal muscle where it involve in anaerobic metabolism and pyruvate reduction (ii) B subunit (LDHB) predominates in cardiac muscle where it helps catalyze aerobic oxidation of pyruvate. Several human cancers such as including renal, breast, gastric, nasopharyngeal cancer etc. have higher LDHA levels compared to normal tissues and LDHA upregulation ensures efficient anaerobic/glycolytic metabolism for tumor cells with reduced oxygen dependency. Moreover, LDHA also plays an important role in the development, invasion and metastasis of several malignancies and induced suppression of LDHA in cancer cells has been shown to trigger ROS burst, mitochondrial pathway apoptosis and limited tumorigenic potential. Defects in LDHA have also been linked to exertional myoglobinuria and glycogen storage disease type 11 (GSD11).
Synonyms	GSD11; HEL-S-133P; LDHM; PIG19
Note	This Lactate Dehydrogenase A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~36 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25004202)
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism

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