GSK3 beta (GSK3B) Mouse Monoclonal Antibody [Clone ID: 3D10]

Specifications

Product Data
Clone Name	3D10
Applications	ELISA, FC, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:200-1:1000, Simple Western: 1:50, Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000
Reactivity	Human, Mouse, Rat, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human GSK3 beta expressed in E. coli. [UniProt# P49841]
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	46 kDa
Gene Name	glycogen synthase kinase 3 beta
Database Link	NP_002084 Entrez Gene 56637 MouseEntrez Gene 84027 RatEntrez Gene 2932 Human UniProt ID P49841
Background	GSK3 (Glycogen synthase kinase 3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK3 are ubiquitously expressed, although particularly high levels of GSK 3 beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK3 alpha and Ser9 of GSK3 beta, S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).
Synonyms	glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta; GSK3beta isoform
Note	This GSK3 beta (3D10) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Alzheimer's disease, Axon guidance, Basal cell carcinoma, B cell receptor signaling pathway, Cell cycle, Chemokine signaling pathway, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Hedgehog signaling pathway, Insulin signaling pathway, Melanogenesis, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, T cell receptor signaling pathway, Wnt signaling pathway

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