NANOG Mouse Monoclonal Antibody [Clone ID: 1E6C4]

Specifications

Product Data
Clone Name	1E6C4
Applications	ELISA, ICC/IF, WB
Recommend Dilution	Western Blot: 1:500-1:2000, ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human Nanog (amino acids 20-166) expressed in E. coli. [UniProt# Q9H9S0]
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ascites
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	35 kDa
Gene Name	Nanog homeobox
Database Link	NP_079141 Entrez Gene 79923 Human UniProt ID Q9H9S0
Background	Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst.
Synonyms	homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
Note	This Nanog (1E6C4) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and ELISA.
Reference Data
Protein Families	Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Stem cell - Pluripotency

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