GAPDH Mouse Monoclonal Antibody [Clone ID: 1A10]

Specifications

Product Data
Clone Name	1A10
Applications	ELISA, FC, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Flow Cytometry: 5 ug/ml, Immunohistochemistry-Paraffin: 1:200-1:1000, Western Blot: 1:500-1:2000, ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Simple Western: 1:50
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human GAPDH expressed in E. coli. [UniProt# P04406]
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	37 kDa
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	NP_002037 Entrez Gene 2597 Human UniProt ID P04406
Background	GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also be reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
Synonyms	G3PD; GAPD; HEL-S-162eP
Note	This GAPDH (1A10) antibody is useful for Western blot, Immunohistochemistry on paraffin embedded sections, Immunocytochemistry/Immunofluorescence and ELISA.
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

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