BIRC5 Mouse Monoclonal Antibody [Clone ID: 8E2]

Specifications

Product Data
Clone Name	8E2
Applications	Block/Neutralize, ELISA, FC, ICC/IF, IHC
Recommend Dilution	Block/Neutralize, ELISA, Flow Cytometry: 1 ug/mL, Flow (Intracellular), Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:50-1:100, Immunohistochemistry-Paraffin: 1:100, In vitro assay
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length recombinant human Survivin [UniProt# O15392]
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	baculoviral IAP repeat containing 5
Database Link	NP_001159 Entrez Gene 64041 RatEntrez Gene 332 Human UniProt ID O15392
Background	Survivin (BIRC5 or IAP4) is the smallest member of inhibitors of apoptosis proteins (X-linked IAP/XIAP, c-IAP1, c-IAP2, IAP-like protein-2/ ILP2, melanoma IAP/ ML-IAP, Livin, NAIP, and survivin) and besides its anti-apoptotic functions; survivin also regulates processes such as cell cycle, migration, metastasis etc. Highly critical in fetal development, survivin is generally no longer present in adult tissues; however, its re-expression occurs in cancers wherein it drives proliferation, metastasis, poor prognosis, and decreased patient survival. Survivin contains only one copy of a modified baculovirus IAP repeat (BIR, a domain required for caspases inactivation) which is used for homodimerization and for its interaction with other chromosome passenger proteins. Phosphorylation/mutation of Thr-48 in the BIR domain affects survivin's dual ability to inhibit apoptosis and regulate cell division. Under apoptotic stimuli involving mitochondria, survivin forms a complex with XIAP, thus increasing the stability of XIAP and its inhibitory activity against caspases. The survivin binding to mitochondrial Smac/DIABLO leads to a delayed Smac/DIABLO release in the cytoplasm resulting in prolonged cell survival. Survivin-HBXIP complex can bind to pro-caspase-9 for exerting prevention of mitochondrial apoptosis activation. For facilitating correct cell division, survivin recognize/complex with phospho-histone-H3 and associates with the chromosome passenger proteins (Aurora B, INCENP, and borealin) and this new complex is recruited to the mitotic centromers where it assists/ensure proper chromosomal segregation. Moreover, at the centrosomes of dividing cells, survivin binds to Cdk1 which is critical for cells to enter mitosis.
Synonyms	API4; EPR-1
Note	This Survivin Antibody (8E2) is useful for Immunocytochemistry, Immunofluorescence and Immunohistochemistry on paraffin embedded sections. For IHC, heat induced antigen retreval using citrate buffer is required. In vitro assay, Blocking/Neutralizing, ELISA, Western Blot, and Flow Cytometry were reported in scientific literature.
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Colorectal cancer, Pathways in cancer

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