Fatty Acid Synthase (FASN) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, IP, WB
Recommend Dilution	Knockdown Validated, Immunohistochemistry: 1:500, Immunocytochemistry/ Immunofluorescence: 1:2000, Immunoprecipitation: 1:100, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:500immunoprecipitation 1:100
Reactivity	Human, Mouse, Rat, Chicken, Hamster, Porcine, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide, conjugated to KLH, made near the N-terminus of mouse FAS. [Swiss-Prot# P19096]
Formulation	Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	272 kDa
Gene Name	fatty acid synthase
Database Link	NP_004095 Entrez Gene 14104 MouseEntrez Gene 50671 RatEntrez Gene 2194 Human UniProt ID P49327
Background	Fatty acid synthetase (FASN) is a key player of fatty acid biosynthetic pathway and it catalyzes formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH (de novo lipid synthesis). Localized in cytoplasm and melanosomes, FASN is expressed at low level in normal adult tissues; however, it is significantly upregulated in cancer cells. The FASN expression is regulated by growth factors/growth factor receptors (EGFR and HER2) and steroid hormone /steroid hormone receptors (ER, AR and progesterone receptor). Downstream of the receptors, PI3K-AKT and MAPK signaling mediate FASN expression through SREBP-1c. FASN expression is also controlled by p53 family proteins and SPOT14, lipogenesis-related nuclear protein generally overexpressed in breast cancer. Extracellular stresses including hypoxia and low pH also induce the FASN expression in cancer cells and USP2a stabilises FASN alongwith promotion of its mTOR-dependent selective translational induction. FASN activation provides rapidly proliferating tumor cells sufficient amount of lipids for membrane biogenesis and confers growth/survival advantage possibly acting as a metabolic oncogene. Because FASN-inhibitors are selectively toxic to tumor cells, it has emerged as an attractive target for cancer.
Synonyms	FAS; OA-519; SDR27X1
Note	This Fatty Acid Synthase antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot where a band at ~272 kDa is observed. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue. In ICC/IF cytoplasmic staining can be seen in MCF7 cells.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Fatty acid biosynthesis, Insulin signaling pathway, Metabolic pathways

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