SCARB1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Block/Neutralize, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution	Flow (Intracellular), Block/Neutralize, Immunohistochemistry-Frozen, Flow Cytometry: 1:400, Immunoblotting, Knockout Validated, Simple Western: 1:100, Western Blot: 1:1000-1:5000, Immunoprecipitation: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:50-1:1000, Immunohistochemistry: 2.5-5 ug/ml, Immunohistochemistry-Paraffin: 2.5-5 ug/ml
Reactivity	Human, Mouse, Rat, Bovine, Chinese Hamster, Mustel
Host	Rabbit
Clonality	Polyclonal
Immunogen	A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt# Q61009]
Formulation	Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	82 kDa
Gene Name	scavenger receptor class B member 1
Database Link	NP_005496 Entrez Gene 20778 MouseEntrez Gene 25073 RatEntrez Gene 949 Human UniProt ID Q8WTV0
Background	SR-BI (scavenger receptor class B member 1, SCARB1) belongs to CD36 family and act as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and HDL located in caveolae (regions over plasma membrane). It plays important role in meditating the uptake of HDL-derived cholesterol as well as cholesteryl ester in liver and steroidogenic tissues. It facilitates the flux of free and esterified cholesterol between cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. SR-BI is expressed in endothelial cells, macrophages and dendritic cells also. It has been suggested to be involved in the phagocytosis of apoptotic cells (via its phosphatidylserine binding activity); uptake of lipid soluble vitamins (vitamin E and carotenoids) and pathogen recognition, anti-inflammatory responses and its expression can be modulated by LPS as well as ROS. SR-BI has also been involved in the capture and cross-presentation of antigens from viruses, bacteria and parasites.
Synonyms	CD36L1; CLA-1; CLA1; HDLQTL6; SR-BI; SRB1
Note	This SR-BI antibody is useful for Flow cytometry (PMID 23029167), Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Immunoprecipitation. In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland.Blocking/Neutralizing was reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24244566)
Reference Data
Protein Families	Druggable Genome, Transmembrane

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