xCT (SLC7A11) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, Immunoblotting, Simple Western, WB
Recommend Dilution	Simple Western, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200, Western Blot: 0.5-2 ug/ml, Flow Cytometry: 1 - 5 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:100 - 1:1000, Immunohistochemistry-Frozen: 1:10-1:500, Immunoblotting
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a region within the N-terminus of the mouse xCT protein (between residues 1-50). [UniProt# Q9WTR6]
Formulation	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	55 kDa
Gene Name	solute carrier family 7 member 11
Database Link	NP_055146 Entrez Gene 23657 Human UniProt ID Q9UPY5
Background	System xc- is a cystine-glutamate exchange transporter that is composed of a light-chain subunit (xCT, SLC7A11) as well as a heavy-chain subunit (CD98hc, SLC3A2), and the xCT expression levels correlate with system xc-activity. SLC7A11 gene encodes xCT which is a multipass membrane protein with 12 transmembrane domains having both termini located intracellularly. xCT is widely expressed in various tissues and exert multiple roles such as pheomelanin production, cellular proliferation/metastasis, Kaposi's sarcoma herpesvirus (KSHV) entry into the host cells, learning and memory. Because cystine absorbed by cells via system xc- is rapidly reduced to cysteine for incorporation into GSH, the cell surface xCT expression is considered an important determinant of intracellular redox balance. Most cells require little cystine uptake to sustain cellular protein biosynthesis and GSH production, however this is different in cancer cells which generate high ROS levels via enhanced metabolism and require high GSH levels to survive oxidative stress. Accordingly, xCT is expressed at high levels in various malignant tumors including leukemias, lymphomas, Karposi's sarcoma, pancreatic cancer, and brain cancer.
Synonyms	CCBR1; xCT
Note	This xCT antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at ~55 kDa; in ICC/IF membrane and ER staining was visualized in HepG2 cells.
Reference Data
Protein Families	Druggable Genome, Transmembrane

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