p53 (TP53) Mouse Monoclonal Antibody [Clone ID: Pab 240]

Specifications

Product Data
Clone Name	Pab 240
Applications	CyTOF-ready, ELISA, FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 1:10-1:1000, ELISA: 1:100-1:2000, Immunohistochemistry: 1:250-1:500, Immunoprecipitation: 10ug/mg, Western Blot: 1:1000-1:2000, Immunohistochemistry-Paraffin: 1:250-1:500, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence: 5 ug/ml, Immunohistochemistry-Frozen, CyTOF-ready
Reactivity	Human, Mouse, Rat, Yeast
Host	Mouse
Clonality	Monoclonal
Immunogen	Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	tumor protein p53
Database Link	NP_000537 Entrez Gene 22059 MouseEntrez Gene 24842 RatEntrez Gene 7157 Human UniProt ID P04637
Background	The tumor suppressor protein p53 plays an important role in cancer development. The p53 protein is a transcription factor that acts as a checkpoint in the cell cycle, either preventing or initiating programmed cell death. Since cancer occurs as the malfunction in the proliferation of cells, disrupting p53 activity allows abnormal cells to multiply or allows a tumor's progression from benign to malignant. This antibody detects p53 in all mammals tested. Detection of p53 using this antibody: 50% of colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissues are negative.
Synonyms	BCC7; LFS1; P53; TRP53
Note	Antigen retrieval with IHC-P is not essential but may optimise staining. In IP this antibody reacts with only mutant p53 protein under non-denaturing conditions. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 1394225) Use in Immunohistochemistry-Frozen reported in various pieces of scientific literature.
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway

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