Apolipoprotein A V (APOA5) Mouse Monoclonal Antibody [Clone ID: 1G5G9]

Specifications

Product Data
Clone Name	1G5G9
Applications	CyTOF-ready, ELISA, FC, IF, IHC, WB
Recommend Dilution	Western Blot: 0.5 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Flow Cytometry: 1 ug per million cells, ELISA: 1:100-1:2000, Immunohistochemistry: 5 ug/ml, ICC/IF (Negative), CyTOF-ready
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Ni-NTA purified full-length recombinant human Apolipoprotein A5 expressed in E. Coli strain BL21 (DE3). [UniProt# Q6Q788]
Formulation	PBS, pH 7.4, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	41 kDa
Gene Name	apolipoprotein A5
Database Link	NP_443200 Entrez Gene 116519 Human UniProt ID Q6Q788
Background	Apolipoprotein A-V (APOA5) belongs to apolipoprotein A1/A4/E family and is a component of several lipoprotein fractions, including VLDL, HDL and chylomicrons. Expressed at high levels in plasma and liver, APOA5 exists as a secreted protein and interacts with GPIHBP1. APOA5 is an important determinant of plasma triglyceride levels by both behaving as a potent stimulator of apo-CII lipoprotein lipase/LPL TG hydrolysis and an inhibitor of hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). APOA5 activates lecithin: cholesterol acyltransferase (LCAT) but does not enhance efflux of cholesterol from macrophages. Transgenic mice overexpressing human APOA5 gene exhibit one-third lower plasma TG levels than wild type, whereas APOA5 knockout mice possess four times as much plasma TG levels, with no effect on plasma cholesterol levels. Clinically, defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR) and hyperlipoproteinemia type 5 (HLPP5).
Synonyms	APOAV; RAP3
Note	This Apolipoprotein A5 antibody is useful in ELISA, Immunohistochemistry paraffin embedded sections and Western blot.
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	PPAR signaling pathway

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