Caspase 1 (CASP1) Mouse Monoclonal Antibody [Clone ID: 14F468]

Specifications

Product Data
Clone Name	14F468
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunohistochemistry-Frozen, Simple Western: 1:50, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin: 1:10-1:500, Immunohistochemistry: 1:100 - 1:500, Western Blot: 0.5-2 ug/ml
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	45 kDa
Gene Name	caspase 1
Database Link	NP_150634 Entrez Gene 12362 MouseEntrez Gene 834 Human UniProt ID P29466
Background	Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.
Synonyms	ICE; IL1BC; P45
Note	Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Reference Data
Protein Families	Druggable Genome, Protease
Protein Pathways	Amyotrophic lateral sclerosis (ALS), Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway

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