D4 (ARHGDIB) Mouse Monoclonal Antibody [Clone ID: 10D774]

Specifications

Product Data
Clone Name	10D774
Applications	FC, ICC/IF, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Flow Cytometry, Western Blot: 1-2 ug/ml
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide corresponding to human D4-GDI/RhoGDI2 was used as the immunogen.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	Rho GDP dissociation inhibitor beta
Database Link	NP_001166 Entrez Gene 397 Human UniProt ID P52566
Background	D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
Synonyms	D4; GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; RhoGDI2
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Neurotrophin signaling pathway

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