ALAS1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, Simple Western, WB
Recommend Dilution	Immunohistochemistry, Western Blot: 1-3 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Simple Western: 1:50
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A portion of amino acids 480-530 of human ALAS-1 were used as the immunogen.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	5'-aminolevulinate synthase 1
Database Link	NP_000679 Entrez Gene 211 Human UniProt ID P13196
Background	Official Gene Symbol: ALAS1 Gene ID: 211(human) Gene Map Locus: 3p21.1(human) ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.
Synonyms	ALAS; ALAS-H; ALAS3; ALASH; MIG4
Reference Data
Protein Pathways	Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism

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