Noxa (PMAIP1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:200, Immunohistochemistry: 1:50 - 1:250, Immunohistochemistry-Paraffin: 1:50 - 1:250, Western Blot: 1 - 2 ug/mL
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody was developed by immunizing rabbits with a mixture of synthetic peptides containing amino acids 51-66 and 75-90 of mouse Noxa.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	phorbol-12-myristate-13-acetate-induced protein 1
Database Link	NP_066950 Entrez Gene 58801 MouseEntrez Gene 492821 RatEntrez Gene 5366 Human UniProt ID Q13794
Background	Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage) (1). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al (1) have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis.
Synonyms	APR; NOXA
Note	Immunohistochemistry-Paraffin: see Inta et al. (2006) for details
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	p53 signaling pathway

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