FANCD2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 5 ug/ml, Chromatin Immunoprecipitation (ChIP), Flow Cytometry: 2 - 5 ug/ml, Simple Western: 1:25, Immunoprecipitation: 1:10-1:500, Immunohistochemistry-Paraffin: 2.5-5.0 ug/ml, Immunohistochemistry: 2.5-5.0 ug/ml, Western Blot: 1 - 2 ug/ml, RNA Inhibition, Immunoblotting, Chromatin Immunoprecipitation, Knockdown Validated, Knockout Validated
Reactivity	Human, Mouse, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	Human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
Formulation	Tris-glycine, 150mM NaCl, pH7.5, 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	Fanconi anemia complementation group D2
Database Link	NP_001018125 Entrez Gene 211651 MouseEntrez Gene 2177 Human UniProt ID Q9BXW9
Background	Fanconi anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation (IR). DNA damage activates the monoubiquitination of FANCD2, targeting nuclear foci containing the BRCA1 protein.
Synonyms	FA-D2; FA4; FACD; FAD; FAD2; FANCD
Note	By Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. For immunofluorescence it has been tested in human MMC and IR treated MEF cells. This antibody may also be used for Immunoprecipitation and Immunohistochemistry-Paraffin
Reference Data
Protein Families	Druggable Genome

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