H3FT (HIST3H3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, Dot, ICC/IF, IHC, WB
Recommend Dilution	WB 1:500 - 1:2000;IF 1:50-1:200;ChIP 1:50 - 1:200
Reactivity	Human, Mouse, Rat, Other (Wide Range)
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Formulation	Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	136
Gene Name	histone cluster 3, H3
Database Link	NP_003484 Entrez Gene 691496 RatEntrez Gene 8290 Human UniProt ID Q16695
Background	Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Synonyms	g; H3; H3.4; H3FT; H3t
Reference Data
Protein Pathways	Systemic lupus erythematosus

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