Scnn1a Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:200-1:2000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)EELDRITEQTLFD, corresponding to amino acid residues 173-185 of rat ENaCa. Extracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	sodium channel epithelial 1 alpha subunit
Database Link	NP_113736 Entrez Gene 6337 HumanEntrez Gene 20276 MouseEntrez Gene 25122 Rat UniProt ID P37089
Background	The DEG/ENaC family consists of Na+ selective channels. They are characterized by 2 transmembrane domains with a large extracellular loop, important for channel activity and intracellular N-and C-termini which are important for channel gating and for interacting with regulatory proteins respectively. ENaC is localized to the apical side of polarized epithelial cells like those in the kidney, lung, and distal colon and is responsible for maintaining proper Na+ balance. This channel is formed by oligomerization of three subunits, α, β, and γ usually with the α2β1γ1 combination. In addition, the a subunit which is essential for channel activity undergoes phosphorylation in its C-terminal by SGK1 (serum and glucocorticoid-induced kinase 1) which is known to activate the channel at the plasma membrane. Pseudohypoaldosteronism type 1 (PHA-1) has an early onset and is depicted by severe dehydration, hyponatremia and hyperkalemia. One form of the pathology is characterized by mutations in α, β, and γ subunits. The severity of the diseases depends on the loss of activity of the channel.
Synonyms	Alpha-ENaC; Alpha-NaCH; BESC2; ENaCa; ENaCalpha; FLJ21883; SCNEA; SCNN1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.