Kcne2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)IVEDWQQKYRSQILHLEDSK, corresponding to amino acid residues 88-107 of rat KCNE2 (MiRP1) .? ? Intracellular, C-terminal part.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	potassium voltage-gated channel subfamily E regulatory subunit 2
Database Link	NP_598287 Entrez Gene 9992 HumanEntrez Gene 246133 MouseEntrez Gene 171138 Rat UniProt ID P63161
Background	KCNE2 (or MiRP1) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. The other members of the family are KCNE1 (IsK, MiNK), KCNE3 (MiRP2), KCNE4 (MiRP3) and KCNE5 (MiRP4). KCNE1 is the founding member of the family and KCNE2 was discovered based on its homology with KCNE1. The KCNE regulatory subunits are small proteins (14- 20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the a subunits. The stoichiometry of the KCNE subunits with their partner a subunits in the native channels is not clear and ratios ranging from 2 to 14 KCNE subunits per 4 a subunits have been proposed. KCNE2 is broadly distributed with prominent expression in brain and heart, but also in other tissues such as skeletal muscle, pancreas and kidney. The best described functional association of KCNE2 is with the voltage-gated K+ channel Kv11.1 (HERG). In the heart, complexes of the two proteins constitute the molecular underlay of the cardiac IKr current and mutations in the KCNE2 subunit are associated with cardiac arrhythmias.KCNE2 can form complexes with other channels including the K+ channels Kv7.1 (KCNQ1) and Kv4.3 and the hyperpolarization-activated cation channels HCN1 and HCN2. In the stomach Kv7.1/KCNE2 complexes were found to be crucial for acid secretion as well as for the maintenance of structural integrity, and for parietal cell survival.
Synonyms	ATFB4; LQT5; LQT6; MGC138292; MIRP1
Reference Data

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