Chrnb4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide CYEGVNILRIPAKR, corresponding to amino acid residues 95-108 of rat nAChRb4 . Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cholinergic receptor nicotinic beta 4 subunit
Database Link	NP_434693 Entrez Gene 1143 HumanEntrez Gene 108015 MouseEntrez Gene 25103 Rat UniProt ID P12392
Background	Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of a subunits (a1-10), which are responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding. The other subunits responsible for making up the active receptor are the Ã? (Ã?1-4), ?, d and e subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore.While most Ã? subunits are neuronal, the Ã?1 subunit forms functional receptors along with other subunits in the muscle. In neurons the a2-a6 and Ã?2-Ã?4 subunits form heteropentameric receptors, usually with a (ax)2(Ã?y)3 stoichiometry.
Synonyms	CHRNB4
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

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