Chrna6 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChRa6. Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cholinergic receptor nicotinic alpha 6 subunit
Database Link	NP_476532 Entrez Gene 11440 MouseEntrez Gene 81721 Rat UniProt ID P43143
Background	Neuronal nicotinic acetylcholine receptors (nAChRs) belong to the superfamily of ligand-gated ion channels and are widely expressed throughout the central and peripheral nervous systems. nAChRs play crucial roles in modulating a wide range of higher cognitive functions by mediating presynaptic, postsynaptic, and extrasynaptic signaling.Nicotinic acetylcholine receptors containing a6 subunits (nAChRa6) exhibit a unique expression pattern in the mammalian brain. nAChRs a6 are abundantly expressed in the midbrain dopaminergic (DAergic) system, including mesocorticolimbic and nigrostriatal pathways, and particularly present in presynaptic nerve terminals. nAChRa6 expressed on DAergic neurons can be activated by endogenous acetylcholine (Ach) or exogenous nicotine and analogs, which suggests that the activation of nAChRa6 may play a vital role in central cholinergic circuits, including modulation of locomotor behaviour and drug addiction. In addition, nAChRa6 is particularly susceptible to nigrostriatal damage, which may lead to Parkinsonâ??s disease (PD). nAChRa6 are also expressed by sensory neurons; therefore, they may also play a role in disorders related to sensory perception.
Synonyms	CHNRA6
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

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