Chrna2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)DLEQMERTVDLKD, corresponding to amino acid residues 189-201 of rat nAChRa2. Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cholinergic receptor nicotinic alpha 2 subunit
Database Link	NP_596911 Entrez Gene 1135 HumanEntrez Gene 110902 MouseEntrez Gene 170945 Rat UniProt ID P12389
Background	Nicotinic Acetylcholine Receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and have longer-term effects on gene expression and cellular connections.nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits, which can be classified as a subunits, containing two cysteine residues at positions analogous to Cys192 and Cys193, and non-alpha subunits (â??structuralâ?? subunits), which can be defined as Ã? subunits when they are expressed in the vertebrate nervous system. There are nine a subunits (a2â??a10) and three Ã? subunits (Ã?2, Ã?3, and Ã?4) in the CNS. Nicotinic receptors are assembled as combinations of a (2-6) and and Ã? (2-4) subunits. All a subunits are expressed in neuronal cells except for the a1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells, as well lymphocytes.In humans, a mutant a2 subunit has been identified, which forms nAChRs with increased agonist sensitivity and causes a form of familial epilepsy6. Further, an a2 subunit mouse model has been used to demonstrate a role for a2 nAChR in nicotine-induced modulation of long-term potentiation in the mouse hippocampal CA1 region, which may underlie some of the cognitive effects of nicotine.
Synonyms	OTTHUMP00000128276
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.