Chrna1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:200-1:2000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide EHETRLVAKLFKD(C), corresponding to amino acid residues 22-34 of rat nAChRa1. Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cholinergic receptor nicotinic alpha 1 subunit
Database Link	NP_077811 Entrez Gene 1134 HumanEntrez Gene 11435 MouseEntrez Gene 79557 Rat UniProt ID P25108
Background	Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of a subunits (a1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding. The other subunits responsible for making up the active receptor are the Ã? (Ã?1-4), ?, d and e subunits. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore.All a subunits are expressed in neuronal cells except for the a1 subunit which is specifically expressed in skeletal muscle. Indeed, two different nAChR structural entities with the following stoichiometry are observed in this tissue: (a1)2Ã?1d? in fetal muscle cells and (a1)2Ã?1de at mature neuromuscular synapses.The a1 extracellular domain contains the main immunogenic region, a region against which a large fraction of autoantibodies against nAChR is directed in the autoimmune disease myasthenia gravis (MG). This autoimmune disease is characterized by the malfunction of neuromuscular transmission as a result of nonfunctional nAChRs, leading to defective signaling at the neuromuscular junction.Due to its central role in muscle contraction and autonomic nervous system, nAChRs have evolved to be important targets of toxins secreted by plants, insects and animals. One prominent toxin affecting a1, in addition to other subunits, is a-bungarotoxin, a snake toxin which blocks nAChRs.
Synonyms	ACHRA; ACHRD; CHNRA; CHRNA; CMS2A; FCCMS; SCCMS
Reference Data

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