Slc6a3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	WB: 1:200-1:2000; FC: 1:50-1:600
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat Dopamine Transporter. Extracellular, 2nd loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	solute carrier family 6 member 3
Database Link	NP_036826 Entrez Gene 13162 MouseEntrez Gene 24898 Rat UniProt ID P23977
Background	The Na+/Cl transporter family, SLC6, includes DAT a monoamine transporter, important for regulating extracellular levels of dopamine. It does so by uptaking dopamine from the synaptic cleft via the co-transport of Na+ and Cl down their electrochemical gradient. The removal of dopamine by DAT remains the most important means to control the extracellular lifetime of the neurotransmitter, and notably the ending of dopaminergic neurotransmission. Transporters for serotonin, norepinephrine, GABA and glycine also belong to this family. These receptors have 12 transmembrane spanning domains and intracellular N- and C-terminal domains. DAT is also subjected to post translational modifications such phosphorylation, important for its regulation. Also, it possesses a large extracellular domain which undergoes N-glycosylation, important for the proper targeting of the transporter to the plasma membrane. DAT is expressed in dopaminergic cell bodies and terminals and can therefore serve as a marker for these neurons. DAT is also expressed in the retina, gastrointestinal tract, lung, kidney, pancreas and lymphocytes. DAT plays an important role in movement as well as reward, learning and memory. Its malfunction which leads to dopaminergic dysregulation has been associated with ADHD, schizophrenia, as well as Parkinsonâ??s disease. DAT is the main target for cocaine and amphetamine and methamphetamine psychostimulants, which mainly increase locomotor activities.
Synonyms	DAT; DAT1
Reference Data

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