Gabra1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABA (A) a1 Receptor. Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	gamma-aminobutyric acid type A receptor alpha1 subunit
Database Link	NP_899155 Entrez Gene 14394 MouseEntrez Gene 29705 Rat UniProt ID P62813
Background	GABA (?-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.3 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.GABAARs are heteropentamers, in which all five subunits contribute to pore formation. To date, eight subunit isoforms have been cloned:a, Ã?, ?, d, e, p, ?, and ?. Six a subunit isoforms have been found to exist in mammals (a1-a6). In most cases, native GABAA receptors consists of 2a, 2Ã?, and 1d subunits. The a subunit is the most common and is expressed ubiquitously. It determines the affinities of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes. The a1 subunit is highly expressed in adulthood while the a2 subunit is highly expressed very early in rat brain development. Failure to complete the normal transition between the a-subunits that are highly expressed in early development (a2, a3, and a5) and those expressed in adulthood (a1) is suggested to play a major role in the development of temporal lobe epilepsy.
Synonyms	ECA4; EJM; EJM5
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

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