Pannexin 2 (PANX2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	WB: 1:200-1:2000; FC: 1:50-1:600
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)EEPIY(S)YTPHNFTRD, corresponding to amino acid residues 76-90 of human Pannexin 2. 1st extracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	pannexin 2
Database Link	NP_443071 Entrez Gene 362979 RatEntrez Gene 406218 MouseEntrez Gene 56666 Human UniProt ID Q96RD6
Background	Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response. Proteins involved in gap junction formation are composed of four transmembrane domains, 2 extracellular loops and one intracellular loop and intracellular N- and C-termini. Several consensus cysteine residues are in the extracellular loop and are essential and necessary for intercellular docking of gap junction hemichannels in the opposing cell membrane. Pannexins (Pannexin 1, Pannexin 2 and Pannexin 3) belong to the superfamily of gap junction proteins. Pannexin 1 (PANX1) is ubiquitously expressed, Pannexin 2 (PANX2) is specifically expressed in the human brain and widespread in rodents and Pannexin 3 (PANX3) is also detected in the brain. The gating properties of Pannexins were studied in Xenopus oocytes and results demonstrate that only PANX1 is able to form homomeric hemichannels, and is also able to form heteromeric hemichannels with PANX2 but not with PANX3. Not surprising, PANX1 gating properties depend whether it forms homomeric or heteromeric hemichannels. A number of different stimuli are known to open these channels and include mechanical stress, extracellular ATP, increases in intracellular Ca2+ and inflammation. Possible roles for Pannexins include paracrine signaling in vascular endothelial cells and taste cell signaling.
Synonyms	hPANX2; PX2
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data
Protein Families	Transmembrane

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.