GRPR Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)RSYHYSEVDTSMLH, corresponding to amino acid residues 287-300 of human BB2. 3rd extracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	gastrin releasing peptide receptor
Database Link	NP_005305 Entrez Gene 14829 MouseEntrez Gene 24938 RatEntrez Gene 2925 Human UniProt ID P30550
Background	Bombesin receptor 2 (BB2) is a member of a family of receptors that binds the 14 amino acid peptide, bombesin. Bombesin was first isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP; a 27 amino acid homologue) and neuromedin B (NMB; a 10 amino acid homologue). BB2 is the preferred receptor for GRP and hence it is also referred to as GRP receptor or GRP-R. BB1 is the preferred receptor for NMB and therefore it is also known as NMB-R. A third member (called BB3) of the bombesin receptor family has been recognized but the endogenous mammalian ligand for this receptor remains to be identified. All three bombesin receptors are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB2 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth. GRP (through its binding to BB2) plays an essential role in the release of hormones in the intestinal tract, the regulation of smooth muscle contraction, and the secretion of pancreatic enzymes. In the brain, BB2 has been implicated in regulating feeding behavior, thermoregulation, and memory formation. Finally, BB2 is overexpressed in several human tumors including breast, prostate, and lung, where it acts as a growth factor receptor inducing tumor growth. BB2 is considered a potential target for the development of both diagnostics and anti-cancer therapies. Alomone labs is pleased to offer a highly specific antibody directed against an epitope located in the 3rd extracellular loop of the human BB2 receptor. Anti-Bombesin Receptor 2 (extracellular) antibody (#ABR-002) can be used in western blot analysis, as well as immunocytochemical and immunohistochemical applications, and will recognize BB2 from human, rat, mouse, and dog samples.
Synonyms	BB2; BB2R
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Calcium signaling pathway, Neuroactive ligand-receptor interaction

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.