Caspase 1 (CASP1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, WB
Recommend Dilution	WB 1:500 - 1:2000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein of human CASP1
Formulation	Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	45 kDa
Gene Name	caspase 1
Database Link	NP_001214 Entrez Gene 12362 MouseEntrez Gene 25166 RatEntrez Gene 834 Human UniProt ID P29466
Background	Caspase-1, or interleukin-1β converting enzyme (ICE/ICEa), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1β and interferon-? inducing factor (IL-18) into their mature forms . Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis . Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock . At least six caspase-1 isoforms have been identified, including caspase-1 a, β, ?, d, e and ? . Most caspase-1 isoforms (a, β, ? and d) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 e typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ? isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the a isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 .
Synonyms	ICE; IL1BC; P45
Reference Data
Protein Families	Druggable Genome, Protease
Protein Pathways	Amyotrophic lateral sclerosis (ALS), Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway

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