NeuN (RBFOX3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 500-2000 WB positive control: Mouse brain tissue lysate IHC: 100-300 Positive control: Human tonsil Predicted cell location: Nucleus and Cytoplasm
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a region derived from 88-102 amino acids of RNA binding protein, fox-1 homolog (C. elegans) 3
Formulation	PBS pH7.3, 0.05% NaN3, 50% glycerol
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	RNA binding protein, fox-1 homolog 3
Database Link	NP_001020619 Entrez Gene 52897 MouseEntrez Gene 146713 Human UniProt ID A6NFN3
Background	Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors); confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA; resulting in nonsense-mediated decay of the message; thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.
Synonyms	Fox-3; Hrnbp3; NeuN; Neun; Neuna60; RGD1560070
Reference Data

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