PARP1 Mouse Monoclonal Antibody [Clone ID: 7A10]

Specifications

Product Data
Clone Name	7A10
Applications	FC, WB
Recommend Dilution	WB: 1:500-2000, IF: 1:200-400
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognizes full-length form (p116) of PARP-1.
Formulation	Supplied in 10 mM HEPES sodium (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol, and 0.03% sodium azide
Purification	N/A
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	poly(ADP-ribose) polymerase 1
Database Link	NP_001609 Entrez Gene 142 Human UniProt ID P09874
Background	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.
Synonyms	ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Base excision repair

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